MOLECULAR BIOLOGICAL METHODS PROGRAMME COURSE DESCRIPTION

Code	Name of the Course Unit	Semester	In-Class Hours (T+P)	Credit	ECTS Credit
TLP204	MOLECULAR BIOLOGICAL METHODS	4	3	2	5

GENERAL INFORMATION
Language of Instruction :	Turkish
Level of the Course Unit :	ASSOCIATE DEGREE, TYY: + 5.Level, EQF-LLL: 5.Level, QF-EHEA: Short Cycle
Type of the Course :	Compulsory
Mode of Delivery of the Course Unit	-
Coordinator of the Course Unit	Assist.Prof. ARZU ÖZGEN
Instructor(s) of the Course Unit	Lecturer ALEYNA ÇAVDAR
Course Prerequisite	No

OBJECTIVES AND CONTENTS
Objectives of the Course Unit:	The application of new techniques in the field of molecular biology
Contents of the Course Unit:	Students will gain the information and application about molecular biology methods

KEY LEARNING OUTCOMES OF THE COURSE UNIT (On successful completion of this course unit, students/learners will or will be able to)
Isolatin of DNA from body fluids and tissues
Prepearing agarose gel electrophoresis
PCR technique
Southern blot analysis
Strip analysis

WEEKLY COURSE CONTENTS AND STUDY MATERIALS FOR PRELIMINARY & FURTHER STUDY
Week	Preparatory	Topics(Subjects)	Method
1	-	General information about molecular methods	-
2	-	Polymerase chain reaction (PCR), real-time PCR and Ribotyping principles	-
3	-	The role and importance of routine laboratory of molecular methods	-
4	-	Microorganisms, human blood and tissue samples for DNA isolation method	-
5	-	Using microorganisms, human blood and tissue samples for DNA isolation method	-
6	-	To prepare PCR, amplification and mixing to ensure distribution	-
7	-	Weigh and resolve agarose, agarose to prepare the tank and pour agarose	-
8	-	MID-TERM EXAM	-
9	-	Agarose gel DNA applique and conduct, view	-
10	-	RFLP mixture to prepare the mixture and make the distribution sector to start	-
11	-	RFLP product to the agarose gel applique and execute, evaluate	-
12	-	RFLP product applique on agarose gel and conduct evaluation	-
13	-	reparing a mixture of multiplex PCR, multiplex PCR amplification mixture and start to make the distribution	-
14	-	reparing a mixture of multiplex PCR, multiplex PCR amplification mixture and start to make the distribution	-
15	-	reparing a mixture of multiplex PCR, multiplex PCR amplification mixture and start to make the distribution	-
16	-	FINAL EXAM	-
17	-	FINAL EXAM	-

SOURCE MATERIALS & RECOMMENDED READING

ASSESSMENT
Assessment & Grading of In-Term Activities	Number of Activities	Degree of Contribution (%)	Description

Level of Contribution
0	1	2	3	4	5

KNOWLEDGE
Theoretical
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	To have adequate infrastructure issues; acquire theoretical and practical knowledge in these areas.						5
2	Required for applications related to the field of methods, techniques and technologies by identifying the services to use effectively.						5

KNOWLEDGE
Factual
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	Basic knowledge and skills acquired in the field, using the ability to interpret and evaluate data, identify problems, analyze the evidence-based solutions to have the ability to develop.				3

SKILLS
Cognitive
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	Required for applications related to the field of modern techniques, tools and informatics technologies to choose and to have knowledge to use it effectively.					4
2	Related to the field of diagnostic data obtained and maintained with the relevant knowledge and skills to have.		1

SKILLS
Practical
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	Required for applications related to the field of modern techniques, tools and informatics technologies to be able to select and use effectively.				3

OCCUPATIONAL
Autonomy & Responsibility
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	To have ability to do individual work.					4
2	Applications related to the field of providing solutions when faced with unforeseen situations.				3

OCCUPATIONAL
Learning to Learn
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	Aware of the necessity of lifelong learning; follow developments in science and technology and continuous self-renewal gain awareness.					4

OCCUPATIONAL
Communication & Social
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	Colleagues, physicians and patients communicate effectively with the ethical framework.	0
2	Public and private sector organizations by examining the processes related to their field skills to practice.	0

OCCUPATIONAL
Occupational and/or Vocational
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	Taking material from the patient for testing, storage and transfer shows sufficient skills.					4
2	Medical laboratory techniques in the materials used and maintained in accordance with the does.					4
3	Required by the field to gain a basic level of computer skills.	0
4	Labor law and occupational safety, environmental protection and quality of information to be conscious.	0

WORKLOAD & ECTS CREDITS OF THE COURSE UNIT
Workload for Learning & Teaching Activities
Type of the Learning Activites	Learning Activities (# of week)	Duration (hours, h)	Workload (h)
Lecture & In-Class Activities	14	3	42
Preliminary & Further Study	14	4	56
Land Surveying	0	0	0
Group Work	0	0	0
Laboratory	0	0	0
Reading	0	0	0
Assignment (Homework)	0	0	0
Project Work	0	0	0
Seminar	0	0	0
Internship	0	0	0
Technical Visit	0	0	0
Web Based Learning	0	0	0
Implementation/Application/Practice	0	0	0
Practice at a workplace	0	0	0
Occupational Activity	0	0	0
Social Activity	0	0	0
Thesis Work	0	0	0
Field Study	0	0	0
Report Writing	0	0	0
Final Exam	1	1	1
Preparation for the Final Exam	0	0	0
Mid-Term Exam	1	1	1
Preparation for the Mid-Term Exam	0	0	0
Short Exam	0	0	0
Preparation for the Short Exam	0	0	0
TOTAL	30	0	100