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MOLEKÜLER BİYOLOJİK YÖNTEMLER COURSE IDENTIFICATION AND APPLICATION INFORMATION

Code Name of the Course Unit Semester In-Class Hours (T+P) Credit ECTS Credit
TLP254 MOLEKÜLER BİYOLOJİK YÖNTEMLER 4 3 3 7

WEEKLY COURSE CONTENTS AND STUDY MATERIALS FOR PRELIMINARY & FURTHER STUDY

Week Preparatory Topics(Subjects) Method
1 - Fundamentals of Molecular Biology – Terms and Concepts -
2 - Fragmentation, Separation, and Purification Methods Used in Molecular Biological Techniques -
3 - DNA Isolation Methods from Microorganisms, Human Blood, and Tissue Samples -
4 - Examples and Comparison of DNA Isolation Methods -
5 - Electrophoresis Methods Using Agarose Gel and Acrylamide Gel -
6 - Gel Interpretation, PCR and Its Variants, and Real-Time PCR -
7 - Real-Time PCR Interpretation and Primer Types -
8 - Restriction Enzymes and the RFLP Method -
9 - RFLP Method Interpretation -
10 - MID-TERM EXAM -
11 - Analysis of Test Samples Using RFLP -
12 - DNA Sequence Analysis -
13 - Examples of Bioinformatics Databases -
14 - DNA Isolation and PCR Applications -
15 - PCR Optimization and Sources of Error -
16 - FINAL EXAM -
17 - FINAL EXAM -