MOLEKÜLER BİYOLOJİK YÖNTEMLER PROGRAMME COURSE DESCRIPTION

Code	Name of the Course Unit	Semester	In-Class Hours (T+P)	Credit	ECTS Credit
TLP254	MOLEKÜLER BİYOLOJİK YÖNTEMLER	4	3	3	7

GENERAL INFORMATION
Language of Instruction :	Turkish
Level of the Course Unit :	ASSOCIATE DEGREE, TYY: + 5.Level, EQF-LLL: 5.Level, QF-EHEA: Short Cycle
Type of the Course :	Compulsory
Mode of Delivery of the Course Unit	-
Coordinator of the Course Unit	Assist.Prof. ARZU ÖZGEN
Instructor(s) of the Course Unit	Lecturer BÜŞRA SARP KAYA-Assist.Prof. İLKSEN SARI O
Course Prerequisite	No

OBJECTIVES AND CONTENTS
Objectives of the Course Unit:	To teach the basic principles of molecular biological methods and the application and interpretation processes of DNA-based analytical techniques.
Contents of the Course Unit:	Course Content: This course covers the basic principles of molecular biological methods, DNA isolation, electrophoresis techniques, PCR and its variants, restriction enzymes, RFLP, DNA sequencing, and the use of bioinformatics databases.

KEY LEARNING OUTCOMES OF THE COURSE UNIT (On successful completion of this course unit, students/learners will or will be able to)
Explains the basic principles of molecular biological methods.
Lists the methods of fragmentation, separation, and purification.
Explains DNA isolation methods from microorganisms, human blood, and tissue samples.
Evaluates the results obtained from agarose and acrylamide gel electrophoresis methods.
Outlines the principles of gel electrophoresis and its main areas of application.
Explains PCR and Real-Time PCR techniques.
Relates primer types to their purposes of use.
Explains restriction enzymes and the RFLP method.
Uses DNA sequencing principles and bioinformatics databases.
Explains the basic steps of DNA isolation and PCR applications.
PCR optimizasyonu sürecini ve deney sırasında ortaya çıkabilecek hata kaynaklarını açıklar.
Explains the PCR optimization process and the potential sources of error that may arise during the experiment.

WEEKLY COURSE CONTENTS AND STUDY MATERIALS FOR PRELIMINARY & FURTHER STUDY
Week	Preparatory	Topics(Subjects)	Method
1	-	Fundamentals of Molecular Biology – Terms and Concepts	-
2	-	Fragmentation, Separation, and Purification Methods Used in Molecular Biological Techniques	-
3	-	DNA Isolation Methods from Microorganisms, Human Blood, and Tissue Samples	-
4	-	Examples and Comparison of DNA Isolation Methods	-
5	-	Electrophoresis Methods Using Agarose Gel and Acrylamide Gel	-
6	-	Gel Interpretation, PCR and Its Variants, and Real-Time PCR	-
7	-	Real-Time PCR Interpretation and Primer Types	-
8	-	Restriction Enzymes and the RFLP Method	-
9	-	RFLP Method Interpretation	-
10	-	MID-TERM EXAM	-
11	-	Analysis of Test Samples Using RFLP	-
12	-	DNA Sequence Analysis	-
13	-	Examples of Bioinformatics Databases	-
14	-	DNA Isolation and PCR Applications	-
15	-	PCR Optimization and Sources of Error	-
16	-	FINAL EXAM	-
17	-	FINAL EXAM	-

SOURCE MATERIALS & RECOMMENDED READING

ASSESSMENT
Assessment & Grading of In-Term Activities	Number of Activities	Degree of Contribution (%)	Description	Examination Method
Mid-Term Exam	1	50
Final Exam	1	50
TOTAL	2	100

Level of Contribution
0	1	2	3	4	5

KNOWLEDGE
Theoretical
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	It explains the basic knowledge in the field of health sciences where the employee will work as a support staff member.				3
2	He/She defines the medical terminology in the field of health sciences, where he/she will work as a support staff member.				3

KNOWLEDGE
Factual
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	They utilize access methods and tools for information and technology related to their field.	0
2	They communicate effectively with patients, colleagues, and other members of the community.				3
3	They become proficient in forming a team and working together with the physician.					4

SKILLS
Cognitive
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1						4
2		0

SKILLS
Practical
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	He/She uses the equipment in his field effectively.				3
2	They effectively use the necessary computer software and hardware in their professional practice.			2

OCCUPATIONAL
Autonomy & Responsibility
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	They can conduct independent research in their field.						5
2	They can find solutions when faced with unforeseen situations in applications related to their field.					4

OCCUPATIONAL
Learning to Learn
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	They are proficient in acquiring the concept of lifelong learning and self-improvement.					4

OCCUPATIONAL
Communication & Social
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	It facilitates teamwork.						5
2	They communicate with colleagues by keeping up with information in their field using a foreign language.			2
3	She/he participates in organizing technical tours.					4

OCCUPATIONAL
Occupational and/or Vocational
	Programme Learning Outcomes	Level of Contribution
		0	1	2	3	4	5
1	They are competent in adopting and applying ethical principles and professional values.						5
2	She/he keeps up with information related to the profession.					4
3	They apply the knowledge and skills they have acquired in hospitals.						5

WORKLOAD & ECTS CREDITS OF THE COURSE UNIT
Workload for Learning & Teaching Activities
Type of the Learning Activites	Learning Activities (# of week)	Duration (hours, h)	Workload (h)
Lecture & In-Class Activities	14	3	42
Preliminary & Further Study	14	2	28
Land Surveying	0	0	0
Group Work	0	0	0
Laboratory	0	0	0
Reading	14	2	28
Assignment (Homework)	6	4	24
Project Work	0	0	0
Seminar	0	0	0
Internship	0	0	0
Technical Visit	0	0	0
Web Based Learning	0	0	0
Implementation/Application/Practice	0	0	0
Practice at a workplace	0	0	0
Occupational Activity	0	0	0
Social Activity	0	0	0
Thesis Work	0	0	0
Field Study	0	0	0
Report Writing	14	2	28
Final Exam	1	2	2
Preparation for the Final Exam	1	15	15
Mid-Term Exam	1	2	2
Preparation for the Mid-Term Exam	1	15	15
Short Exam	0	0	0
Preparation for the Short Exam	0	0	0
TOTAL	66	0	184