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MOLEKÜLER BİYOLOJİK YÖNTEMLER COURSE IDENTIFICATION AND APPLICATION INFORMATION

Code Name of the Course Unit Semester In-Class Hours (T+P) Credit ECTS Credit
TLP254 MOLEKÜLER BİYOLOJİK YÖNTEMLER 4 3 3 7

KEY LEARNING OUTCOMES OF THE COURSE UNIT (On successful completion of this course unit, students/learners will or will be able to)

Explains the basic principles of molecular biological methods.
Lists the methods of fragmentation, separation, and purification.
Explains DNA isolation methods from microorganisms, human blood, and tissue samples.
Evaluates the results obtained from agarose and acrylamide gel electrophoresis methods.
Outlines the principles of gel electrophoresis and its main areas of application.
Explains PCR and Real-Time PCR techniques.
Relates primer types to their purposes of use.
Explains restriction enzymes and the RFLP method.
Uses DNA sequencing principles and bioinformatics databases.
Explains the basic steps of DNA isolation and PCR applications.
PCR optimizasyonu sürecini ve deney sırasında ortaya çıkabilecek hata kaynaklarını açıklar.
Explains the PCR optimization process and the potential sources of error that may arise during the experiment.